Part:BBa_K5194000:Design
pSB1K0 Yeast Shuttle Vector (sfGFP dropout) Type IIS
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 41 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 41
Illegal NheI site found at 1371
Illegal NheI site found at 2498
Illegal NheI site found at 2521
Illegal NotI site found at 3502 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 41
Illegal BglII site found at 154
Illegal BamHI site found at 69
Illegal XhoI site found at 4555 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 41 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 41
Illegal NgoMIV site found at 4968
Illegal NgoMIV site found at 5094
Illegal NgoMIV site found at 5104
Illegal AgeI site found at 231
Illegal AgeI site found at 5350 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 3436
Illegal BsaI.rc site found at 2466
Illegal SapI site found at 4625
Illegal SapI site found at 4996
Illegal SapI.rc site found at 2577
Design Notes
The backbone was designed to be compatible with the open yeast collection for both level 1 and level 2 assemblies. It includes cut sites around the E. coli selection marker, allowing it to be swapped out, which makes the backbone distinguishable between level 1 and level 2 assemblies. The plasmid has a GFP dropout, which allows for visualization of successful assemblies.
Source
The genomic sequence comes from Komagataella pastoris and Pseudomonas aeruginosa.
References
The design was inspired by the JUMP vector collection. Marcos Valenzuela-Ortega, Christopher French, Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology, Synthetic Biology, Volume 6, Issue 1, 2021, ysab003, https://doi.org/10.1093/synbio/ysab003